SHANDONG BIO&MEDIA LABORATORIES CO., LTD.  

Product Name: Alkaline Phosphatase (ALP) Assay Kit/Test Kit/Ivd Reagents Model NO.: ALP Type: Alp Assay Kit Quality Guarantee Period: 16 Months Application: Hospital, Lab, School Classification: Biological Diagnostics Certification: ISO13485 Group: All Groups Trademark: Biomedia Transport Package: Foam Box Specification: R1: 2*40 mL, R2: 1*20 mL Origin: Jinan, Shandong Product Description The alkaline phosphatase (ALP) assay kit is intended for the accurate and direct quantitative measurement of ALP activity in human plasma. ALP measurement is useful in the diagnosis of liver, bile diseases and bone disorders.Test principle4-Nitrophenyl Phosphate (4-NPP) can be dephosphorylated by ALP and converted into 4- Nitrophenol (4-NP), which turns yellow due to it quinoid structure formed in alkaline environment. The unit activity of ALP can be calculated from the increasing rate of absorbance detected at a wavelength of 405 nm.Product Features1. Linear range of ALP activity: [3, 500] U/LLinear correlation coefficient ∣r∣≥ 0.990Linear deviation ≤ ± 10.00%2. Absorbance of blank ≤ 0.803. Changing rate of absorbance of blank ΔA/min ≤ 0.05 4. SensitivityChanging rate of absorbance of blank ΔmA/min ≥ 0.05 while testing the specific ALP sample of activity of 223 U/L.5. AccuracyRelative deviation is within ±10%.6. PrecisionIntra CV% ≤ 6.0%; inter CV% ≤8.0%.Technical ParametersTemperature37ºCOptical path length (OPL)1.0 cmMain wavelength405 nmSecondary wavelength480 nm (Optional)MethodVelocity methodReaction directionPositiveSample4 μLR1200 μLR250 μLDelay time50 sReaction time180 sReference range of ALP activity: 3 - 110 U/L.Packing SpecificationsR1: 2×40 mL, R2: 1×20 mLR1: 4×60 mL, R2: 4×15 mLR1: 1×40 mL, R2: 1×10 mL (Sample)Storage and stabilityUnopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 15 months).Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.Do not freeze the assay kit.